![]() ![]() The Tag2 insertion adjacent to RFL1 was unique to the Ler-0 ecotype, however, and was not present in two other ecotypes that lack RPS5. Southern hybridization analysis of six Arabidopsis ecotypes revealed 4–11 Tag2-homologous sequences in each, indicating that this element is ubiquitous in Arabidopsis and has been active in recent evolutionary time. Sequence analysis of the RPS5 deletion region in Ler-0 revealed the presence of an Ac-like transposable element, which we have designated Tag2. The Ler-0 ecotype contains RFL1, but lacks RPS5. The RPS5 and RFL1 disease resistance genes of Arabidopsis ecotype Col-0 are oriented in tandem and are separated by 1.4 kb. Whereas members within one class are greater than 95% identical to each other, the DNA sequence identity between the two classes is only approximately 70%. Direct sequencing of the 5′ end of these PCR products, and phylogenetic analysis of the sequence data, confirmed the presence of the two distinct classes ofcpsC. ![]() Long-range PCR was used to amplify thecps regions between cpsB and aliAfrom a variety of pneumococcal serotypes. pneumoniae cps loci can be divided into two distinct classes. That is, all serotypes tested contained high-stringency homologues of either the cps19aC to -E genes or thecps19fC to -E genes, but not both. pneumoniaeserotypes with probes specific for the cps19aC,cps19aD, and cps19aE genes indicated a hybridization pattern complementary to that previously reported forcps19fC, cps19fD, and cps19fE. Southern hybridization analysis of the cps loci from various S. The former genes were designatedcps19aA to -G and were 70 to 90% identical to their cps19f counterparts. We suggest that pRHL2 has invertron termini, as has been reported previously for Streptomyces linear replicons.Īnalysis of the sequence data obtained from the 5′ portion of theStreptococcus pneumoniae type 19A capsular polysaccharide biosynthesis locus (cps19a) revealed that the first seven genes are homologous to the first seven genes in the type 19F (cps19f) locus. Retardation of both terminal fragments in the gel shift assay indicated that each terminus of pRHL2 is linked to a protein. Southern hybridization analysis with pRHL2 terminal probes suggested that the right terminus of pRHL2 is similar to pRHL1 and pRH元 termini. The left and right termini of pRHL2 had 3-bp perfect terminal inverted repeats and were not as similar to each other (64% identity) as the known actinomycete linear replicons are. The termini of pRHL2 were cloned and sequenced. These results suggested that the linear plasmid is a possible determinant of propagation of the diverse degradative genes in rhodococci. Conjugal transfer of pRHL2 between RHA1 mutant derivatives was observed at a frequency of 7.5 × 10−5 transconjugant per recipient. We constructed a physical map of pRHL2, and the degradative enzyme genes, including bphB2, etbD2, etbC, bphDEF, bphC2, and bphC4, were localized in three regions. In this study, the structural and functional characteristics of pRHL2 were determined. The diverse degradative genes are distributed mainly on the pRHL1 and pRHL2 plasmids. strain RHA1, has diverse biphenyl/PCB degradative genes and harbors huge linear plasmids, including pRHL1 (1,100 kb), pRHL2 (450 kb), and pRH元 (330 kb). A strong polychlorinated biphenyl (PCB) degrader,Rhodococcus sp.
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